GBI – Genera Biosystems Ltd.

Advanced Molecular Diagnostic Systems – Multiplex qPCR

A novel multiplex qPCR method for assessing the comparative lengths of telomeres

The comparative size of telomeres is taken into account to be associated to ailments comparable to most cancers, ageing, and cardiovascular ailments. qPCR is at the moment one of many primary strategies for detecting telomere size. Nonetheless, as a result of distinctive sequence of telomeres (extremely repetitive six-base sequence), it's tough to design primers and probes to broaden and detect telomere and to place inner reference gene and...

Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR

The warmth-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in uncooked milk, is a serious explanation for destabilization and untimely spoilage of ultra-high temperature (UHT) milk and milk merchandise. To allow fast detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in uncooked milk, we...

Development of two specific multiplex qPCRs to determine amounts of Pseudomonas, Enterobacteriaceae, Brochothrix thermosphacta and Staphylococcus in meat and heat-treated meat products

Culturing strategies are conventionally utilized to analyze the contamination of meals with a number of microorganisms after warmth processing. Nonetheless, with these strategies, it's not doable to guage whether or not heat-treated meat merchandise, similar to cooked sausages, contained elements of spoiled meat. Subsequently, two particular multiplex qPCRs have been developed on this research as a way to decide the microbiological high quality...

Multiplex qPCR Assay for Direct Detection and Quantification of Colletotrichum truncatum, Corynespora cassiicola, and Sclerotinia sclerotiorum in Soybean Seeds

Exact analysis of plant illnesses is without doubt one of the best instruments to attenuate yield losses. Colletotrichum truncatum, Corynespora cassiicola, and Sclerotinia sclerotiorum are frequent soilborne pathogens that have an effect on soybeans everywhere in the world. We developed a multiplex quantitative real-time polymerase chain response (qPCR) assay to concurrently detect and quantify the three pathogens in soybean seeds and to...