GBI – Genera Biosystems Ltd.

Advanced Molecular Diagnostic Systems – Multiplex qPCR

Development of two specific multiplex qPCRs to determine amounts of Pseudomonas, Enterobacteriaceae, Brochothrix thermosphacta and Staphylococcus in meat and heat-treated meat products

Development of two specific multiplex qPCRs to determine amounts of Pseudomonas, Enterobacteriaceae, Brochothrix thermosphacta and Staphylococcus in meat and heat-treated meat products

Culturing strategies are conventionally utilized to analyze the contamination of meals with a number of microorganisms after warmth processing. Nonetheless, with these strategies, it’s not doable to guage whether or not heat-treated meat merchandise, similar to cooked sausages, contained elements of spoiled meat. Subsequently, two particular multiplex qPCRs have been developed on this research as a way to decide the microbiological high quality of the uncooked supplies used for these merchandise. The PCR targets targeted on 4 bacterial teams usually discovered on meat (household Enterobacteriaceae, genus Pseudomonas, genus Staphylococcus and species Brochothrix thermosphacta).

Specificity in addition to sensitivity of the developed multiplex qPCRs, validated through the use of 68 microbial species, have been 100%. The applicability of each multiplex qPCRs in comparison with culturing strategies was carried out utilizing 96 meat samples (contemporary and naturally spoiled) and 12 inhouse-made “Lyoner” sausages containing variable ratios of spoiled meat (0%, 5%, 12% and 25%; n = Three for every group). Each strategies confirmed comparable outcomes by evaluating the ∆log10 cfu/g, the relative accuracy and the t-test evaluation (p > 0.05).

Evaluating qPCR outcomes of the totally different sausage teams, a major distinction between sausages containing contemporary meat and sausages containing spoiled meat (12% and 25%) was discovered just for Pseudomonas and B. thermosphacta in each uncooked and cooked sausages. The statistical distinction between 5% vs. 12% and 25% spoiled meat in cooked sausages, was additionally discovered just for these two bacterial teams. The developed multiplex qPCRs have been additional utilized to 30 commercially accessible “Bologna-type” sausages. The outcomes confirmed a complete of 14 sausages thought-about to be suspicious for Meals Fraud.

Whereas the function of Staphylococcus spp. in meat spoilage stays unclear, Pseudomonas, Enterobacteriaceae and B. thermosphacta may collectively be used as an indicator for “spoiled meat” utilized in sausages. The developed qPCR methods on this research permit the detection of 4 related bacterial teams within the heated Bologna-type sausages and supply details about the hygienic high quality of uncooked supplies used. This methodology may thus be useful for screening meals suspected of Meals Fraud.

Proof for variations within the temporal progress of Plasmopara viticola clades riparia and aestivalis airborne inoculum monitored in vineyards in jap Canada utilizing a selected multiplex qPCR assay

4 clades of Plasmopara viticola remoted from wild and cultivated Vitis species have just lately been described. Presently, solely P. viticola clades riparia and aestivalis had been detected in jap Canada. With a purpose to improve our understanding of the epidemiology of those clades of P. viticola, airborne sporangia have been monitored with spore samplers at two experimental vineyards from 2015 to 2018, and at 11, 14, and 15 industrial vineyards in 2016, 2017, and 2018, respectively. At every winery and in annually, airborne sporangia have been assessed thrice weekly from grapevine budbreak to reap.

To precisely monitor airborne inoculum, a selected and delicate qPCR assay for simultaneous quantification of P. viticola clades riparia and aestivalis was developed. On the experimental web site, within the winery planted with the hybrid grape selection Chancellor, principally P. viticola clade riparia was detected. In vineyards planted with a number of grape varieties, airborne sporangia of P. viticola clade riparia have been prevalent at first of the season, whereas P. viticola clade aestivalis was principally detected from mid-season to reap. On the industrial websites, airborne sporangia of P. viticola clade riparia have been extra prevalent in 2016, whereas P. viticola clade aestivalis was extra prevalent in 2017 and 2018.

Development of two specific multiplex qPCRs to determine amounts of Pseudomonas, Enterobacteriaceae, Brochothrix thermosphacta and Staphylococcus in meat and heat-treated meat products

The one vital distinction between the inoculum progress curves was the time at which 50% of the seasonal inoculum was reached, with a median for the three years of 100.eight and 117.9 days since Might 1 for P. viticola clade riparia and clade aestivalis, respectively. When airborne sporangium concentrations have been expressed because the proportion of the 2 clades, basically, the proportion of clade aestivalis to that of riparia was low at first of the season and elevated to succeed in round 0.9 to 1.Zero on the finish of the season.

Multiplex-invasive reaction-assisted qPCR for quantitatively detecting the abundance of EGFR exon 19 deletions in cfDNA

Exon 19 deletions (19-Del) on the epidermal development issue receptor (EGFR) gene are important biomarkers for guiding tyrosine kinase inhibitor (TKI) remedy and the prognosis of non-small cell lung most cancers (NSCLC). Nonetheless, it’s tough for typical qPCR to quantitatively detect all 19-Del targets of EGFR, particularly for cfDNA samples. Herein, a multiplex invasive reaction-assisted qPCR was proposed by using a multiplex invasive response to differentiate 19-Del DNA targets from wild DNA targets and report them with totally different fluorescence indicators in every PCR cycle.

As all 19-Del targets have the identical amplification effectivity and really comparable invasive response efficiencies, the 19-Del abundance in a pattern could possibly be quantified through the use of the distinction between the Ct values (ΔCt) of the deletion targets and the wild targets with out the requirement of a normal calibration curve. Combining the excessive sensitivity of PCR and the excessive specificity of the invasive response, this methodology can detect 10 copies of the deletion targets and decrease than 0.1% deletion abundance.

Green qPCR SuperMix UDG

20-abx098032
  • EUR 1135.00
  • EUR 453.00
  • EUR 662.00
  • 15 ml
  • 1 ml
  • 5 ml

Top Green qPCR SuperMix

20-abx098033
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix

20-abx098034
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Library Quantification qPCR SuperMix

20-abx098896
  • EUR 230.00
  • EUR 411.00
  • 1 ml
  • 5 ml

Pro QPCR SuperMix Kit - ROX premixed

K5056200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit - ROX premixed

K5056400 400 reactions
EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054200 200 reactions
EUR 408
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054400 400 reactions
EUR 594
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 211
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Top Green qPCR SuperMix (+Dye II)

20-abx098890
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix (+Dye II)

20-abx098891
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 718

HiScript II Q RT SuperMix for qPCR

R222-01 100 rxn (20 μl/rxn)
EUR 235

HiScript II Q Select RT SuperMix for qPCR

R232-01 100 rxn (20 μl/rxn)
EUR 235

HiScript III RT SuperMix for qPCR (+g DNA wiper)

R323-01 100 rxn (20 μl/rxn)
EUR 293

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)

R233-01 100 rxn (20 μl/rxn)
EUR 244

PCR SuperMix

20-abx098887
  • EUR 272.00
  • EUR 578.00
  • 1 ml
  • 5 ml

electrophoresis power supply

EV245 ea
EUR 479

CyanRed Power Box

2305226 2unit
EUR 283
Description: i ndepent l y use for CyanRed Epi

Power Broth™

106 500 g
EUR 103

Power Broth™

117 500 ml
EUR 63

PCR SuperMix (-dye)

20-abx098002
  • EUR 411.00
  • EUR 189.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 5 ml

PCR SuperMix (+dye)

20-abx098003
  • EUR 411.00
  • EUR 189.00
  • EUR 6174.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 480 ml
  • 5 ml

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Plus PCR SuperMix

20-abx098888
  • EUR 244.00
  • EUR 467.00
  • 1 ml
  • 5 ml

Library Amplification SuperMix

20-abx098894
  • EUR 258.00
  • EUR 537.00
  • 1 ml
  • 5 ml

OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 89

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 141

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

Entrans qPCR Probe Kit

RK21209 40 RXN Ask for price

qPCR Control Template Kit

G929 100 rxns
EUR 148

Lentivirus qPCR Quantification Kit

K1471-100 100 Rxns
EUR 298

Retrovirus qPCR Quantification Kit

K1472-100 100 Rxns
EUR 318

QPCR Positive Control Kit

M1126-100
EUR 403

COLLECTION CONTAINER 1L, EVA FILM

91-200-01 1/pk
EUR 80
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 2L, EVA FILM

91-200-02 1/pk
EUR 88
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 5L, EVA FILM

91-200-05 1/pk
EUR 99
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-10 1/pk
EUR 101
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-20 1/pk
EUR 110
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-36 1/pk
EUR 86
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-39 1/pk
EUR 89
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 1L, EVA FILM

91-200-42 1/pk
EUR 73
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 5L, EVA FILM

91-200-43 1/pk
EUR 151
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-45 1/pk
EUR 126
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-47 1/pk
EUR 132
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 50L, EVA FILM

91-200-48 1/pk
EUR 146
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 100L, EVA FILM

91-200-82 1/pk
EUR 279
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 200L, EVA FILM

91-200-83 1/pk
EUR 292
Description: Flexible Containers; Bioprocess Bags and Parts

Alkaline phosphatase Power AP

AZ140A 1MG
EUR 100.46

Alkaline phosphatase Power AP

AZ235A 1MG
EUR 116.12

Four Source Power Supply 

EDK621 1 UNIT
EUR 684.23

Power Prime Broth™

112 500 g
EUR 111

Power Prime Broth™

122 500 ml
EUR 64

Power Prime-olate™

162 500 g
EUR 106

Power Prime-olate™

172 500 ml
EUR 68

12v vehicle power adapter

BSH100-A12 1 PC
EUR 429.53

T PCR SuperMix (-dye)

20-abx098005
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml

T PCR SuperMix (+dye)

20-abx098006
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml

EasyPfu PCR SuperMix (-dye)

20-abx098009
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml

FastPfu PCR SuperMix (-dye)

20-abx098010
  • EUR 244.00
  • EUR 495.00
  • 1 ml
  • 5 ml

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

Built in power supply for WSE-1165 Power AC 100-240V

WSE-650-659 1unit
EUR 726
Description: Assembl y power suppl y wi t h WSE-1165 such as bui l t -i n

Methylamp MS-qPCR Fast Kit

P-1028 200 Reactions
EUR 496.6
Description: fast delivery possible

Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
EUR 276.2

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

COLLECTION CONTAINER 500 ML, EVA FILM

91-200-41 1/pk
EUR 69
Description: Flexible Containers; Bioprocess Bags and Parts

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 850

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

electrophoresis power supply uk plug

EV245-UK ea
EUR 479

electrophoresis power supply 120 vac

EV245-US ea
EUR 479

Dual Source 150 Power Supply 

EDK620 1 UNIT
EUR 371.46

Power leads for HorizeBLOT-R

2322462 5unit
EUR 270

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 411.00
  • EUR 189.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 5 ml

Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
EUR 850

igScript RT-qPCR Kit - 50 Reactions

4513 1/EA
EUR 248

Coronavirus Rapid RT-qPCR Detection Kit

K1461-100 100 Rxns
EUR 551

Adeno-Associated Virus qPCR Quantification Kit

K1473-100 100 Rxns
EUR 348

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110

OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit

STA-859 200 assays
EUR 508
Description: OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit is a quantitative assay for measuring the antioxidant potential within various samples. Following the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the sample, the kit colorimetric probe develops a blue color that is read colorimetrically at 540-600nm. The antioxidant potential of samples is determined based on an iron standard curve and results are calculated at Fe2+ equivalents (µM) or FRAP value.

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 3831.4

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 3831.4

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 3831.4

Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric)

K515-200
EUR 550

The outcomes have been 100% in line with ARMS-PCR for the 38 tumor tissues examined and have been in good settlement with next-generation sequencing for quantifying the abundance of EGFR 19-Del in 15 cfDNA samples, displaying the nice potential of the tactic for liquid biopsies. These outcomes counsel that each clades of P. viticola coexist, however that clade aestivalis is predominant and that downy mildew epidemics attributable to P. viticola clade riparia happen 2-Three weeks earlier than these attributable to clade aestivalis.

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