Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR
The warmth-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in uncooked milk, is a serious explanation for destabilization and untimely spoilage of ultra-high temperature (UHT) milk and milk merchandise. To allow fast detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in uncooked milk, we...