The power to detect and quantify aerially dispersed plant pathogens is crucial for growing efficient illness management measures and epidemiological fashions that optimize the timing for management . There’s an acute want for managing the downy mildew pathogens infecting cucurbits and hop incited by members of the genus Pseudoperonospora (P. cubensis clade 1 and a couple of isolates and P. humuli, respectively). A extremely particular multiplex TaqMan qPCR assay concentrating on distinctive sequences within the pathogens’ mitochondrial genomes was developed that permits detection of all three taxa in a single multiplexed amplification.
An inner management included within the response evaluated if outcomes have been influenced by PCR inhibitors that may make it by way of the DNA extraction course of. Dependable quantification of inoculum as little as three sporangia in a pattern was noticed. The multiplexed assay was examined with DNA extracted from purified sporangia, contaminated plant tissue and environmental samples collected on impaction spore traps samplers. The power to precisely detect and concurrently quantify all three pathogens in a single multiplexed amplification ought to enhance administration choices for controlling the ailments they trigger.
A selected area of Cm (clvG gene) was chosen for primer design utilizing comparative genomics strategy. A completely artificial common inner management (UIC) was additionally designed to detect PCR inhibitors and false detrimental leads to qPCR reactions. The Cm primers can be utilized alone or in a triplex TaqMan qPCR assay with UIC and beforehand described Clavibacter-specific primers. The assay was particular for Cm and detected upto 10 fg of Cm DNA in sensitivity and spiked assay. Addition of the UIC didn’t change the specificity or sensitivity of the multiplex TaqMan qPCR assay.
Particular and Delicate Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR
mRNA RT-qPCR is proven to be a really delicate method to detect minimal residual illness (MRD) in sufferers with neuroblastoma. A number of mRNA markers are identified to detect heterogeneous neuroblastoma cells in bone marrow (BM) or blood from sufferers. Nevertheless, the restricted volumes of BM and blood obtainable can hamper the detection of a number of markers. To make optimum use of those samples, we developed a multiplex RT-qPCR for the detection of MRD in neuroblastoma. GUSB and PHOX2B have been examined as single markers. The adrenergic markers TH, GAP43, CHRNA3 and DBH and mesenchymal markers POSTN, PRRX1 and FMO3 have been examined in multiplex. Utilizing management blood and BM, we established new thresholds for positivity.
Comparability of multiplex and singleplex RT-qPCR outcomes from 21 blood and 24 BM samples from neuroblastoma sufferers demonstrated a comparable sensitivity. With this multiplex RT-qPCR, we’re capable of check seven totally different neuroblastoma mRNA markers, which overcomes tumor heterogeneity and improves sensitivity of MRD detection, even in these samples of low RNA amount. With sources and time being saved, discount in pattern quantity and consumables can help within the introduction of MRD by RT-qPCR into scientific follow. The outcomes demonstrated the potential of quantifying as few as 2.5 copies/response and evaluation of 244 sufferers with identified outcomes chosen by cross-section revealed 100% settlement with a qualitative RT-qPCR assay registered by Anvisa. On this inhabitants, it was doable to quantify sufferers with between 2.59 and three.5 × 107 copies per response and detrimental sufferers continued to point the identical end result.
The MRD disk: automated minimal residual illness monitoring by extremely delicate centrifugal microfluidic multiplex qPCR
We current a proof-of-principle research on automated, extremely delicate and multiplexed qPCR quantification by centrifugal microfluidics. The MRD disk can be utilized for standardisation of repetitive, longitudinal assays with excessive necessities on reproducibility and sensitivity, equivalent to most cancers monitoring. In distinction to high-throughput qPCR automation by cumbersome and costly robotic workstations we make use of a small centrifugal microfluidic instrument, addressing the necessity of low- to mid-throughput purposes. As a possible utility we display automated minimal residual illness (MRD) monitoring of prognostic markers in sufferers with acute lymphoblastic leukaemia (ALL).
The disk-workflow covers all features of scientific gold customary MRD quantification: era of normal curves, specificity controls, no template controls and quantification of the ALL affected person pattern. We built-in a extremely delicate, colorimetric 2-plex evaluation of MRD targets, in addition to a 2-plex evaluation of reference genes, each in parallel and in a single LabDisk cartridge. For this function, a scientific process for crosstalk- and signal-to-noise-optimisation is launched, offering a suggestion for environment friendly multiplex readout inside microfluidic platforms. The qPCR customary curves (n = 12/12) generated on-disk attain clinically required linearity (R2 = 98.1% to R2 = 99.8%).
In three consecutive MRD disk runs with an ALL affected person pattern containing the 2 consultant MRD targets VH3D3D5JH3 and VkIkde, we observe excessive accordance between the on-disk quantifications (48 ± 6 copies/response and 69 ± 6 copies/response) and the anticipated concentrations (57 copies/response for each targets). Compared to the scientific gold customary of manually pipetted, singleplex assays, the MRD disk yields comparable restrict of quantification (1 × 10-4) in n = 6/6 analyses (vs. n = 4/Four in gold customary) and a restrict of detection (1 × 10-5) in n = 6/6 evaluation (vs. n = 2/Four in gold customary).
 Pro QPCR SuperMix Kit |
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| K5053200 | Biochain | 200 reactions | EUR 188 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Pro QPCR SuperMix Kit |
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| K5053400 | Biochain | 400 reactions | EUR 299 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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 Eva QPCR SuperMix Kit |
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| K5052200 | Biochain | 200 reactions | EUR 211 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Eva QPCR SuperMix Kit |
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| K5052400 | Biochain | 400 reactions | EUR 320 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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 Green qPCR SuperMix |
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| 20-abx098031 | Abbexa |
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 Probe qPCR SuperMix |
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| 20-abx098040 | Abbexa |
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 Power Eva QPCR SuperMix Kit |
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| K5057200 | Biochain | 200 reactions | EUR 188 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Power Eva QPCR SuperMix Kit |
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| K5057400 | Biochain | 400 reactions | EUR 320 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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 Green qPCR SuperMix UDG |
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| 20-abx098032 | Abbexa |
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 Top Green qPCR SuperMix |
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| 20-abx098033 | Abbexa |
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 Tip Green qPCR SuperMix |
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| 20-abx098034 | Abbexa |
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 Library Quantification qPCR SuperMix |
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| 20-abx098896 | Abbexa |
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 cDNA Synthesis SuperMix for qPCR |
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| 20-abx09801920ulSystems | Abbexa |
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) T-Pro Fast Blocking Buffer (in PBS) |
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| JK92-W001 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
) T-Pro Fast Blocking Buffer (in TBS) |
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| JK92-W002 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
 Methylamp MS-qPCR Fast Kit |
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| P-1028 | EpiGentek | 200 Reactions | EUR 496.6 |
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Description: fast delivery possible |
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) Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
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| 31014 | Biotium | 2x1mL | EUR 228 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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) Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
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| 31014-1 | Biotium | 5x1mL | EUR 437 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
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| 31014-2 | Biotium | 50x1mL | EUR 3576 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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) Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
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| 31015-1 | Biotium | 5x1mL | EUR 437 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
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| 31015-2 | Biotium | 50x1mL | EUR 3576 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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) Top Green qPCR SuperMix (+Dye II) |
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| 20-abx098890 | Abbexa |
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) Tip Green qPCR SuperMix (+Dye II) |
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| 20-abx098891 | Abbexa |
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) VAHTS Library Quantification Kit for Illumina (Low ROX Premixed) |
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| NQ103 | Vazyme | 500 rxn | EUR 291 |
) VAHTS Library Quantification Kit for Illumina (High ROX Premixed) |
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| NQ104 | Vazyme | 500 rxn | EUR 291 |
) HotTaq Probe qPCR Mix (ROX) |
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| BT11001 | Bioatlas | 250rxn | EUR 109 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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) HotTaq EvaGreen qPCR Mix (ROX) |
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| BT11101 | Bioatlas | 250rxn | EUR 123 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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 TaqProbe 5X qPCR MasterMix-ROX |
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| MasterMix-5P | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 213 |
 BrightGreen 5X qPCR MasterMix-ROX |
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| MasterMix-5R | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 213 |
 KiloGreen 2X qPCR MasterMix-ROX |
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| MasterMix-KR | ABM | 4 x 1.25 ml - 500 reactions (20 ul) | EUR 140 |
 BrightGreen miRNA qPCR MasterMix-ROX |
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| MasterMix-mR | ABM | 4 x 1.25 ml for 500 reactions | EUR 151 |
 TaqProbe 2X qPCR MasterMix-ROX |
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| MasterMix-P | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
 BrightGreen 2X qPCR MasterMix-ROX |
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| MasterMix-R | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
BrightGreen 2X qPCR MasterMix-ROX |
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| MasterMix-R-XL | ABM | 16 x 1.25 ml for 2000 reactions (20 ul) | EUR 376 |
: (1mL)) Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
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| 31014-T | Biotium | 1mL | EUR 134 |
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Description: Minimum order quantity: 1 unit of 1mL |
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) Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
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| 31015 | Biotium | 2x1mL | EUR 228 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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: (1mL)) Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
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| 31015-T | Biotium | 1mL | EUR 134 |
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Description: Minimum order quantity: 1 unit of 1mL |
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) cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-50rxns20ulSystems | Abbexa | 50 rxns × 20 ul Systems | EUR 718 |
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 HiScript II Q RT SuperMix for qPCR |
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| R222-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 235 |
) HotTaq Probe qPCR Mix (no ROX) |
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| BT11002 | Bioatlas | 250rxn | EUR 109 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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) HotTaq EvaGreen qPCR Mix (no ROX) |
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| BT11102 | Bioatlas | 250rxn | EUR 123 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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 BrightGreen 5X qPCR MasterMix-Low ROX |
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| MasterMix-5L | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 213 |
 TaqProbe 5X qPCR MasterMix-Low ROX |
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| MasterMix-5PL | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 213 |
 BrightGreen Express 2X qPCR MasterMix-ROX |
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| MasterMix-ER | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
 KiloGreen 2X qPCR MasterMix-Low ROX |
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| MasterMix-KL | ABM | 4 x 1.25 ml - 500 reactions (20 ul) | EUR 140 |
 BrightGreen 2X qPCR MasterMix-Low ROX |
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| MasterMix-LR | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
BrightGreen 2X qPCR MasterMix-Low ROX |
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| MasterMix-LR-XL | ABM | 16 x 1.25 ml for 2000 reactions (20 ul) | EUR 376 |
 BrightGreen miRNA qPCR MasterMix-Low ROX |
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| MasterMix-mL | ABM | 4 x 1.25 ml for 500 reactions | EUR 151 |
 TaqProbe 2X qPCR MasterMix-Low ROX |
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| MasterMix-PL | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
 HiScript II Q Select RT SuperMix for qPCR |
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| R232-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 235 |
 ROX reference dye for qPCR, 500 uL |
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| 31110 | lumiprobe | 500 uL | EUR 46 |
 ROX reference dye for qPCR, 1 mL |
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| 41110 | lumiprobe | 1 mL | EUR 52 |
) SYBR Green qPCR Master Mix (High ROX) |
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| HY-K0521 | MedChemExpress | 1 mL (100 rxns) | EUR 113 |
) SYBR Green qPCR Master Mix (Low ROX) |
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| HY-K0522 | MedChemExpress | 5 mL (500 rxns ) | EUR 257 |
) SYBR Green qPCR Master Mix (No ROX) |
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| HY-K0523 | MedChemExpress | 5 mL (500 rxns ) | EUR 257 |
 BrightGreen Express 2X qPCR MasterMix-Low ROX |
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| MasterMix-EL | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
 Accuris qMax Probe No Rox qPCR Mix |
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| PR2001-N-1000 | BenchMark | 1 PC | EUR 467.74 |
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) HiScript III RT SuperMix for qPCR (+g DNA wiper) |
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| R323-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 293 |
 PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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| HS-100-QPCR | CORNING | 100/pk | EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
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 PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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| HS-150-QPCR | CORNING | 100/pk | EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
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 QCell-Pro One-Step qRT-PCR SuperMix Kit |
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| K5055200 | Biochain | 200 reactions | EUR 376 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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QCell-Pro One-Step qRT-PCR SuperMix Kit |
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| K5055400 | Biochain | 400 reactions | EUR 572 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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) 2x SYBR Green qPCR Master Mix (High ROX) |
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| B21402 | Bimake | 5 ml | EUR 224 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21403 | Bimake | 25 ml | EUR 856 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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) 2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21702 | Bimake | 5 ml | EUR 224 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21703 | Bimake | 25 ml | EUR 856 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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) Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
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| QPCR001-R | Bio Basic | 4X1.25ml | EUR 252.71 |
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 and Fast ligase (2.5ul)]) Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
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| CAS9LIG-KIT | SBI | 1 Kit | EUR 153 |
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) Fast EvaGreen Master Mix for qPCR(200 rxn) |
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| 31003 | Biotium | 2x1ML | EUR 204 |
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Description: Minimum order quantity: 1 unit of 2x1ML |
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) Fast EvaGreen Master Mix for qPCR (500 rxn) |
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| 31003-1 | Biotium | 5x1ML | EUR 386 |
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Description: Minimum order quantity: 1 unit of 5x1ML |
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) Fast EvaGreen Master Mix for qPCR (5000 rxn) |
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| 31003-2 | Biotium | 50x1ML | EUR 3116 |
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Description: Minimum order quantity: 1 unit of 50x1ML |
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) Fast Plus EvaGreen qPCR Master Mix (200 rxn) |
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| 31020 | Biotium | 2x1mL | EUR 228 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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) Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
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| 31020-1 | Biotium | 5x1mL | EUR 437 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
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| 31020-2 | Biotium | 50x1mL | EUR 3576 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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 AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn |
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| AZ-2301 | Real Time Primers | 200 Rxn | EUR 179 |
 AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn |
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| AZ-2305 | Real Time Primers | 500 Rxn | EUR 320 |
 AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn |
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| AZ-2320 | Real Time Primers | 2000 Rxn | EUR 996 |
 AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn |
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| AZ-2325 | Real Time Primers | 5000 Rxn | EUR 2348 |
 AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn |
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| AZ-2401 | Real Time Primers | 200 Rxn | EUR 179 |
 AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn |
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| AZ-2405 | Real Time Primers | 500 Rxn | EUR 320 |
 AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn |
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| AZ-2420 | Real Time Primers | 2000 Rxn | EUR 996 |
 AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn |
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| AZ-2421 | Real Time Primers | 5000 Rxn | EUR 2348 |
) Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
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| QPCR002-L | Bio Basic | 4X1.25ml | EUR 252.71 |
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) Fast Probe Master Mix with Rox (200 rxn) |
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| 31016 | Biotium | 2x1mL | EUR 234 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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) Fast Probe Master Mix with Rox (500 rxn) |
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| 31016-1 | Biotium | 5x1mL | EUR 466 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Fast Probe Master Mix with Rox (5000 rxn) |
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| 31016-2 | Biotium | 50x1mL | EUR 3871 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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) HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
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| R233-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 244 |
 T-Pro BCA Protein Assay kit |
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| JB04-D001 | T-Pro Biotechnology | 500assay/KIT | EUR 204 |
) T-Pro Plasmid Mini Kit (100) |
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| RB94-YPD100 | T-Pro Biotechnology | 100preps/Kit | EUR 161 |
) T-Pro Plasmid Mini Kit (250) |
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| RB94-YPD250 | T-Pro Biotechnology | 250preps/Kit | EUR 222 |
) T-Pro Plasmid Midi Kit (20) |
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| RB94-YPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 213 |
) T-Pro Plasmid Maxi Kit (10) |
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| RB94-YPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 222 |
) Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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| W2601-1 | 101Bio | NULL | EUR 0 |
Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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| W2601-5 | 101Bio | NULL | EUR 0 |
) T-Pro Bradford Protein Assay kit(1X) |
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| JB04-D002 | T-Pro Biotechnology | 500ml/BT | EUR 161 |
) T-Pro Genomic DNA Midi Kit (20) |
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| RB94-NGM020 | T-Pro Biotechnology | 20preps/kit | EUR 222 |
) T-Pro Genomic DNA Mini Kit (100) |
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| RB94-NGS100 | T-Pro Biotechnology | 100preps/kit | EUR 213 |
 T-Pro Stacking Buffer |
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| JB03-C002 | T-Pro Biotechnology | 500ml/BT | EUR 152 |
 T-Pro MycoClean spray |
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| JT90-R002 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
 PCR SuperMix |
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| 20-abx098887 | Abbexa |
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) T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K002M | T-Pro Biotechnology | 250ml*2/Kit | EUR 204 |
T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K002S | T-Pro Biotechnology | 100ml*2/Kit | EUR 152 |
) T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K004M | T-Pro Biotechnology | 250ml*2/Kit | EUR 239 |
T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K004S | T-Pro Biotechnology | 100ml*2/Kit | EUR 170 |
) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
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| 31042-1 | Biotium | 5x1mL | EUR 272 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
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| 31042-20mL | Biotium | 2x10mL | EUR 494 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
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| 31042-T | Biotium | 1mL | EUR 114 |
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Description: Minimum order quantity: 1 unit of 1mL |
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 (500 reactions)) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
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| 9-31045 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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 (500 reactions)) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
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| 9-31046 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Fast EvaGreen Master Mix for qPCR, trial size (100 rxn) |
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| 31003-T | Biotium | 1ML | EUR 123 |
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Description: Minimum order quantity: 1 unit of 1ML |
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) Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn) |
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| 31020-T | Biotium | 1mL | EUR 134 |
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Description: Minimum order quantity: 1 unit of 1mL |
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) T-Pro Endotoxin Removal Plasmid Midi kit (25) |
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| RB94-EPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 248 |
) T-Pro Endotoxin Removal Plasmid Maxi kit (10) |
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| RB94-EPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 265 |
) T-Pro Plant Genomic DNA Midi Kit (20) |
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| RB94-PGM020 | T-Pro Biotechnology | 20preps/kit | EUR 222 |
) T-Pro Plant Genomic DNA Mini Kit (100) |
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| RB94-PGS100 | T-Pro Biotechnology | 100preps/kit | EUR 222 |
) Fast Probe Master Mix with Rox, trial size (100 rxn) |
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| 31016-T | Biotium | 1mL | EUR 141 |
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Description: Minimum order quantity: 1 unit of 1mL |
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) T-Pro Gel/PCR DNA Purification Maxi Kit (10) |
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| RB94-NEL010 | T-Pro Biotechnology | 10preps/Kit | EUR 161 |
) T-Pro Gel/PCR DNA Purification Mini Kit (100) |
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| RB94-NES100 | T-Pro Biotechnology | 100preps/Kit | EUR 161 |
) T-Pro Gel/PCR DNA Purification Mini Kit (250) |
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| RB94-NES250 | T-Pro Biotechnology | 250preps/Kit | EUR 222 |
 T-Pro EZ Gel Solution 8% |
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| JB02-B008M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 8% |
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| JB02-B008S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
 T-Pro EZ Gel Solution 10% |
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| JB02-B010M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 10% |
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| JB02-B010S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
 T-Pro EZ Gel Solution 12% |
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| JB02-B012M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 12% |
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| JB02-B012S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
 T-Pro EZ Gel Solution 15% |
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| JB02-B015M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 15% |
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| JB02-B015S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
 T-Pro Separating or Resolving Buffer |
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| JB03-C001 | T-Pro Biotechnology | 500ml/BT | EUR 152 |
) T-Pro Transfer Blotting buffer (10X) |
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| JB08-H001 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
 T-Pro Western Blot Stripping Reagent |
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| JB11-K002 | T-Pro Biotechnology | 500ml/BT | EUR 178 |
) T-Pro Aqua EZ Clean (M) |
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| JT90-R001M | T-Pro Biotechnology | 500ml/BT | EUR 332 |
) T-Pro Aqua EZ Clean (S) |
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| JT90-R001S | T-Pro Biotechnology | 100ml/BT | EUR 151 |
 T-Pro EZ stain Gel solution |
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| JT90-R005M | T-Pro Biotechnology | 500ml/BT | EUR 187 |
T-Pro EZ stain Gel solution |
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| JT90-R005S | T-Pro Biotechnology | 100ml/BT | EUR 126 |
 T-Pro P-Fect Transfection Reagent |
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| JT97-N005M | T-Pro Biotechnology | 1.0ml/vial | EUR 222 |
) PCR SuperMix (-dye) |
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| 20-abx098002 | Abbexa |
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) PCR SuperMix (+dye) |
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| 20-abx098003 | Abbexa |
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 cDNA Synthesis SuperMix |
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| 20-abx09801420ulSystems | Abbexa |
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 Plus PCR SuperMix |
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| 20-abx098888 | Abbexa |
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 Library Amplification SuperMix |
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| 20-abx098894 | Abbexa |
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 OneScriptcDNA Synthesis SuperMix |
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| G451 | ABM | 25 x 20 ul reactions | EUR 89 |
OneScriptcDNA Synthesis SuperMix |
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| G452 | ABM | 100 x 20 ul reactions | EUR 141 |
 Evo? cDNA Supermix |
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| M1168-100 | Biovision | EUR 381 | |
Evo? cDNA Supermix |
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| M1168-25 | Biovision | EUR 267 | |
 Novo? cDNA Supermix |
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| M1169-100 | Biovision | EUR 441 | |
Novo? cDNA Supermix |
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| M1169-25 | Biovision | EUR 289 | |
 T-Pro BCA Protein Assay Reagent A |
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| JB04-D001A | T-Pro Biotechnology | 500ml/BT | EUR 170 |
 T-Pro BCA Protein Assay Reagent B |
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| JB04-D001B | T-Pro Biotechnology | 12ml/BT | EUR 117 |
 qPCR Control Template Kit |
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| G929 | ABM | 100 rxns | EUR 148 |
 QPCR Positive Control Kit |
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| M1126-100 | Biovision | EUR 403 | |
 Lentivirus qPCR Quantification Kit |
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| K1471-100 | Biovision | 100 Rxns | EUR 298 |
 Retrovirus qPCR Quantification Kit |
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| K1472-100 | Biovision | 100 Rxns | Ask for price |
 Reagent (reducing 4X)) T-Pro Laemmli (SDS sample) Reagent (reducing 4X) |
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| JB06-F002 | T-Pro Biotechnology | 10ml/BT | EUR 126 |
) T-Pro Tris-Glycine-SDS running buffer (10X) |
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| JB07-G001 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
) T-Pro Tris-Glycine-Native running buffer (10X) |
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| JB07-G002 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
) T-Pro Semi Dry Transfer Blotting buffer (10X) |
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| JB08-H002 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
) T-Pro Protein Free Blocking Buffer (in PBS) |
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| JK92-W003 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
) T-Pro Protein Free Blocking Buffer (in TBS) |
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| JK92-W004 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
) T-Pro Nonliposomal Transfection Reagent I (NTR I) |
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| JT97-N001M | T-Pro Biotechnology | 1.0ml/vial | EUR 187 |
) T-Pro Nonliposomal Transfection Reagent II (NTR II) |
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| JT97-N002M | T-Pro Biotechnology | 1.0ml/vial | EUR 204 |
) T-Pro Nonliposomal Transfection Reagent III (NTR III) |
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| JT97-N006M | T-Pro Biotechnology | 1.0ml/vial | EUR 222 |
 Jade? QRT Kit-ROX |
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| M1178-100 | Biovision | EUR 370 | |
 Taqman QRT Kit-ROX |
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| M1186-100 | Biovision | EUR 370 | |
 5-ROX |
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| B8212-10 | ApexBio | 10 mg | EUR 284 |
5-ROX |
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| B8212-5 | ApexBio | 5 mg | EUR 195 |
5-ROX |
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| B8212-50 | ApexBio | 50 mg | EUR 773 |
 6-ROX |
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| HY-D0053 | MedChemExpress | 5mg | EUR 187 |
5-ROX |
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| HY-D0784 | MedChemExpress | 50mg | EUR 601 |
, packed in 1,25 ml vials) qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S210 | GeneOn | 100 rcs (2,5 ml) | EUR 138 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S210L | GeneOn | 500 rcs (12,5 ml) | EUR 523 |
 Reagent (non-reducing 4X)) T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X) |
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| JB06-F003 | T-Pro Biotechnology | 10ml/BT | EUR 126 |
) T PCR SuperMix (-dye) |
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| 20-abx098005 | Abbexa |
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) T PCR SuperMix (+dye) |
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| 20-abx098006 | Abbexa |
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) EasyPfu PCR SuperMix (-dye) |
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| 20-abx098009 | Abbexa |
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) FastPfu PCR SuperMix (-dye) |
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| 20-abx098010 | Abbexa |
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 OneScriptPlus cDNA Synthesis SuperMix |
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| G453 | ABM | 25 x 20 ul reactions | EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G454 | ABM | 100 x 20 ul reactions | EUR 169 |
 AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS |
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| AP-STR-KIT-P | CORNING | 1/pk | EUR 721 |
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Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
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) T-Pro Washing buffer in PBS and Tween-20 (10X) |
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| JB09-I003 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
) T-Pro Washing buffer in TBS and Tween-20 (10X) |
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| JB09-I004 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
The automation reduces the chance of handbook liquid dealing with errors, making the MRD disk a gorgeous resolution to guarantee reproducibility in moderate-throughput, longitudinal gene quantification purposes.